- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078mg/ml
- 国产
- ELA-E0931h
- 2025年07月02日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
phospho Protein Kinase C
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078mg/ml
- 规格：
96T
Human phospho Protein Kinase C ELISA Kit
Product Name:Human phospho Protein Kinase C ELISA Kit
Packing:96T
Catalog No.:ELA-E0931h
Gene Name:P-PKC
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:phospho Protein Kinase C
Alternative Name:P-PKC,phospho Protein Kinase C
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human phospho Protein Kinase C ELISA Kit allows for the in vitro quantitative determination of phospho Protein Kinase C concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human phospho Protein Kinase C ELISA Kit has been pre-coated with an phospho Protein Kinase C antibody specific to phospho Protein Kinase C .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for phospho Protein Kinase C and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain phospho Protein Kinase C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of phospho Protein Kinase C in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Translocation Assays of Protein Kinase C Activation
The protein kinase C (PKC) family members include at least 11 different isoforms that, based on their different requirements for activation, have been divided into three subfamilies, the Ca2+ -dependent (cPKCα, (βI , βII
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
: phospho?motif antibody; phosphorylation; protein kinase GO TO THE FULL PROTOCOL: PDF or HTML at Wiley Online Library
Protein Kinase C as an Effector of Lipid-Derived Second Messengers
the activation and function of protein kinase C in these immune cells. Since neutrophils provide a ready source of human primary tissue, these methods are also useful for pharmacological studies on the protein kinase C system and for evaluation of protein kinase
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