- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078mg/ml
- 国产
- ELA-E0927h
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Histamine
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078mg/ml
- 规格：
96T
Human Histamine, HIS ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Histamine,;
Search name
Human Histamine ELISA Kit, Human HIS ELISA Kit, Histamine ELISA Kit, HIS ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of general histamine,Histamine concentrations in serum, plasma,
tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Histamine, During the reaction, Histamine in the sample or standard competes with a fixed amount
of biotin-labeled Histamine for sites on a pre-coated Monoclonal antibody specific to Histamine. Excess conjugate and unbound sample or
standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and
incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a
sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of
Histamine in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Warnings And Limitations This product is for research use only. Not intended for any animal or human therapeutic or diagnostic use unless otherwise stated. Follow appropriate laboratory guidelines. This product contains 0.02% sodium azide
Mast Cell Histamine and Cytokine Assays
RI) on cord bloodderived mast cells by myeloma IgE and anti-human IgE is used to induce histamine release. Histamine levels were measured in the culture supernatants using an enzymelinked immunosorbent assay. A human mast cell line (HMC-1), derived
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人组胺 ( Histamine )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 Histamine 单抗包被于酶标板上，标准品和样品中的 Histamine与单抗结合，加入
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