- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078mg/ml
- 国产
- ELA-E0701h
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
8- epi- prostaglandin F2alpha, 8- epi- PGF2Alpha
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078mg/ml
- 规格：
96T
Human 8- epi- prostaglandin F2alpha, 8- epi- PGF2Alpha ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
8-epi-prostaglandin F2alpha(8-epi-PGF2α) ,8-Isoprostane
Search name
Human 8-Isoprostane ELISA kit,Human 8-epi-PGF2α ELISA Kit鈥? Human 8-epi-prostaglandin F2alpha ELISA Kit, Human 8-epi-PGF2 alpha ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human 8-epi-prostaglandin F2alpha,8-epi-PGF2α concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 8-epi-PGF2α, During the reaction, 8-epi-PGF2α in the sample or standard competes with a fixed amount of biotin-labeled 8-epi-PGF2α for sites on a pre-coated Monoclonal antibody specific to 8-epi-PGF2α. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 8-epi-PGF2α in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验and are subsequently released in the free form following cleavage by phospholipase A2 (3 ). The presence of F2 -IPs in vivo was initially reported by Morrow et al. (1 ). The levels of one of the abundant isomers, 8-epi-PGF2α , was shown to increase by up to 200-fold
Oxford Biomedical Research的氧化应激及慢性炎症生物标志物检测
as an index of oxidative stress in vivo, Free Radic. Biol. Med. 28:505-513 (2000).? 2. 15-异前列烷F2t ELISA分析试剂盒（血清、血浆、尿液和组织），货号EA84，96孔板：检测血清、血浆、尿液和组织中15-异前列烷F2t水平。 注：15-异前列烷F2t（15-Isoprostane F2t）别名：8-表-前列腺素F2α/8-异前列腺素F2α/ 8-异前列烷（8-epi-PGF2α/8-iso-PGF2
receptor antibody 乙酰胆碱受体抗体 ACIF anti-complement immunofluorescent method 抗补体免疫荧光法 α-1-ACt alpha(α)-1-anti-chymotrypsin α-1-抗糜蛋白酶 ACTH adrenocorticotropin hormone 促肾上腺皮质激素 AD Alzheimer's disease 老年性痴呆 ADDC antibody dependent
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