- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078mg/ml
- 国产(香港）
- ELA-E0590h
- 2026年05月21日
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- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
keratan sulfate
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078mg/ml
- 规格：
96T
Human Keratan sulfate, KS ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Keratan sulfate, keratosulfate,KS
Search name
Human Keratan sulfate ELISA Kit, Human KS ELISA Kit, Human keratosulfate ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human keratan sulfate,Keratan sulfate concentrations in serum,
plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Keratan sulfate, During the reaction, Keratan sulfate in the sample or standard competes with a
fixed amount of biotin-labeled Keratan sulfate for sites on a pre-coated Monoclonal antibody specific to Keratan sulfate. Excess conjugate
and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each
microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the
addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The
concentration of Keratan sulfate in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
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文献和实验Isolation and Characterization of Glycosaminoglycans from Human Atheromatous Vessels
either in free form or attached to a protein core to form proteoglycans (3 ). On the basis of their composition, GAGs are grouped into four major categories: hyaluronic acid, heparin and heparan sulfate, chondroitin and dermatan sulfates, and keratan sulfate
调至 1μg/μl。 4. ELISA 检测：按试剂盒说明书完成板平衡、加样孵育、洗板、酶标抗体孵育、显色终止与读数，结合标准曲线得出 IFN-γ 浓度。 四、操作要点速记 1.组织样本新鲜处理，全程低温操作，裂解液现配现用； 2. 蛋白定量时标准品与样本同步孵育，稀释采用倍比稀释减少误差； 3. ELISA 实验需每次重绘标准曲线，洗板充分、严格把控显色与终止时间。 免疫检测试剂盒： 货号-规格 名称 abs510001-96T Human IL-2 ELISA Kit (Faster
Assay for Human Rad51-Mediated DNA Displacement Loop Formation
DNA (Stratagene) Plasmid Midi kit (QIAGEN) Proteinase K (Roche Applied Science) Recombinant Hop2-Mnd1 complex, purified (5 µM) or recombinant human Rad54, purified (5 µM) (see Step 5) Purify Hop2-Mnd1 complex
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