Human EGR1 ELISA Kit
Product Name:Human EGR1 ELISA Kit
Packing:96T
Catalog No.:ELA-E0416h
Gene Name:EGR1
Detect Range:0.156-10 ng/mL
Sensitivity:0.078-10 ng/mL
Target Protein Name:Human Early growth response protein 1
Alternative Name:Early growth response protein 1, EGR-1, AT225, Nerve growth factor-induced protein A, NGFI-A, Transcription factor ETR103, Transcription factor Zif268, Zinc finger protein 225, Zinc finger protein
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human EGR1 ELISA Kit allows for the in vitro quantitative determination of Human Early growth response protein 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human EGR1 ELISA Kit has been pre-coated with an EGR1 antibody specific to Human Early growth response protein 1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Early growth response protein 1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Early growth response protein 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm