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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.31-20ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
MCAb
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1ng/ml
- 规格:
96T
Human MCAb ELISA Kit
Product Name:Human MCAb ELISA Kit
Packing:96T
Catalog No.:ELA-E0390h
Gene Name:MCAb
Detect Range:15.6-1000 U/mL
Sensitivity:7.80 U/mL
Target Protein Name:Human Melanocyte antibody
Alternative Name:Melanocyte antibody, MCAb
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human MCAb ELISA Kit allows for the in vitro quantitative determination of Human Melanocyte antibody concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human MCAb ELISA Kit has been pre-coated with an MCAb antibody specific to Human Melanocyte antibody .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Melanocyte antibody and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Melanocyte antibody, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm
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文献和实验Warnings And Limitations This product is for research use only. Not intended for any animal or human therapeutic or diagnostic use unless otherwise stated. Follow appropriate laboratory guidelines. This product contains 0.02% sodium azide
以分泌特异性抗体的人B 淋巴细胞与瘤细胞融合而成的杂交瘤细胞所分泌的抗体称为人单克隆抗体(human McAb,HMcAb)。与MMcAb 相比,HMcAb 的优越性在于其应用于人体内不会激起明显快速的抗McAb 反应。HMcAb 在人体内的滞留时间长,并能更有效地与人体免疫系统协同发挥作用。HMcAb 的制备方法包括:人-鼠细胞杂交技术;人-人细胞杂交技术;淋巴细胞EB 病毒转化-杂交技术及基因工程等等。 HMcAb 制备的技术线路如图22
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
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