Target Protein Name:Human Interleukin-27 subunit alpha
Alternative Name:Interleukin-27 subunit alpha, IL-27 subunit alpha, IL-27-A, IL27-A, p28, IL27, IL27A Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human IL27 ELISA Kit allows for the in vitro quantitative determination of Human Interleukin-27 subunit alpha concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human IL27 ELISA Kit has been pre-coated with an IL27 antibody specific to Human Interleukin-27 subunit alpha .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Interleukin-27 subunit alpha and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Interleukin-27 subunit alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm