- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078mg/ml
- 国产
- ELA-E0381h
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Factor VIII
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078mg/ml
- 规格：
96T
Human Factor VIII ELISA Kit
Product Name:Human Factor VIII ELISA Kit
Packing:96T
Catalog No.:ELA-E0381h
Gene Name:FVIII
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Factor VIII
Alternative Name:FVIII,Factor VIII
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Factor VIII ELISA Kit allows for the in vitro quantitative determination of Factor VIII concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Factor VIII ELISA Kit has been pre-coated with an Factor VIII antibody specific to Factor VIII .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Factor VIII and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Factor VIII, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Factor VIII in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Identification of Mutations in the Human Factor VII Gene
It has been recognized from the early 1800s that activation of coagulation can be initiated by the exposure of subendothelial layers (tissue factor), but it was the 1940s before factor VII (FVII) was included in this event
Use of Human Hepatocytes to Investigate Blood Coagulation Factor
is to describe experimental procedures allowing to measure the secretion by primary human hepatocytes and functional activity (including production of fibrillar material from extracellular medium) of haemostasis proteins including factors II, V, VII, VIII, PIVKA-II (protein
Rinse with PSA 3x 0.3% hydrogen peroxide in PBS, 30min. Then rinse in PBS 3x 5% normal goad serum in PBS at RT for 1 hour. Rabbit anti-Human Factor VIII Related Antigen diluted to 1:500 with 1% NGS in PBS, incubated
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