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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Angiotensin IV, ANGIV
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078mg/ml
- 规格:
96T
Human Angiotensin IV, ANGIV ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Human ANGIV ELISA Kit, Human Angiotensin IV ELISA Kit, Human ANG-IV ELISA Kit, Human ANG IV ELISA Kit
Intended use
Human ANGIV ELISA kit allows for the in vitro quantitative determination of Human Angiotensin IV concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
Human ANGIV ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ANGIV, During the reaction, ANGIV in the sample or standard competes with a fixed amount of biotin-labeled ANGIV for sites on a pre-coated Monoclonal antibody specific to ANGIV. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ANGIV in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human ANGIV ELISA kit Range 0.15-10ng/ml
ANGIV Related Products
> ANGIV ELISA KITS
Bovine Angiotensin IV, ANGIV ELISA Kit
Canine Angiotensin IV, ANGIV ELISA Kit
Chicken Angiotensin IV, ANGIV ELISA Kit
Human Angiotensin IV, ANGIV ELISA Kit
Mouse Angiotensin IV, ANGIV ELISA Kit
Porcine Angiotensin IV, ANGIV ELISA Kit
Rabbit Angiotensin IV, ANGIV ELISA Kit
Rat Angiotensin IV, ANGIV ELISA Kit
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文献和实验Activation and Expansion of Human Treg Cells
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有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂,增加了操作步骤,在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法:1, TORCH及传染病试剂盒(间接法),见2.2.32, TORCH-IgM捕获法特色:包被抗体,标记抗原原理:3, 细胞因子试剂盒采用的方法路线(ABC-ELISA)原理产品特色:采用ABC法,灵敏度更高,特异性更强。生物素抗体和酶联物是浓缩的,使用前需用相应的缓冲液稀释。酶联物可以通用
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