- ￥3500
- GeneTex
- 美国
- GTX00422-pro
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
- 保质期：
12 months from the shipping date of the product.
- 英文名：
Human EPO protein, His and GST tag (active)
- 库存：
Available
- 供应商：
GeneTex
- 规格：
10 μg
Cell proliferation effect of GTX00422-pro Human EPO protein (active), cell viability was measured by cell Counting Kit-8 (CCK-8).
(A) Unstimulated TF-1 cells cultured in RPMI-1640 for 72hrs.
(B) TF-1 cells cultured in RPMI-1640, stimulated with 5 ng/ml EPO for 72hrs.
Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human EPO protein, His and GST tag (active) (GTX00422-pro) (500-7.81 ng/mL). Coated protein was probed with EPO antibody (GTX100813) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.
SDS-PAGE analysis of GTX00422-pro Human EPO protein (active).
WB analysis of GTX00422-pro Human EPO protein (active).
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文献和实验GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
Brigham & Women's Hospital, Boston, MA 02115 Excerpted from Protein: Protein Interactions, Second Edition Edited by Erica A. Golemis and Peter D. Adams ABSTRACT Glutathione-S-Transferase (GST) fusion
Purification of Baculovirus-Expressed Human DNA Topoisomerase I
of human topo I (htopo I) in this manner becomes both costly and laborintensive if milligram quantities are needed for structural studies. Although active htopo I has been overexpressed in mammalian cells (3 ), yeast (4 ), and Escherichia coli
Purification of Human Multiprotein Complexes using OneSTrEP Technology
sufficient for visualising single protein bands by Coomassie Blue staining (Figure 1). The Strep -tag®II (SAWRHPQFGG) and its “double” sister, the One-STrEP-tag (tandem arrangement of Strep -tag®II, here called OneStrep), are reasonably small protein
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