蛋白别名 HSPC150 antibody, PIG50 antibody, UBE2T antibody, Ubiquitin carrier protein T antibody, Ubiquitin protein ligase T
蛋白介绍
The ubiquitin (Ub)-mediated protein degradation pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub to specific protein substrates. The first step requires ATP-dependent activation of the C-terminus of Ub and the assembly of multi-Ubs by Ub-activating enzyme E1. The ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain, is then conjugated to Ubs, through a thiol-ester linkage between a conserved cysteine and the C-terminus of Ub, to generate an intermediate Ub-E2 complex. Then the E3, a ligase, catalyzes the transfer of Ub from E2 to the appropriate substrate. This pathway regulates many fundamental cellular processes. There are also other E2s which form thiol-ester linkages without the use of E3s as well as several UBC homologs (TSG101, Mms2, Croc-1 and similar proteins), which lack the active site cysteine essential for ubiquitination and appear to function in DNA repair pathways.
产品描述
anti-UBE2T/HSPC150 antibody is a Rabbit Polyclonal antibody againstUBE2T/HSPC150..
建议稀释比例
WB : 1:200-1:2000;IP:1:200-1:2000;IF:1:50-1:500
IHC
Western blot
Jurkat cells were subjected to SDS PAGE followed by western blot with PAab09185(UBE2T antibody) at dilution of 1:800(本抗体仅供体外科研用途,不可用于临床诊断!)