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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
signal transducer and activator of transcription 3 (acute-phase response factor)
- 亚型:
IgG
- 形态:
liquid
- 保存条件:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
- 克隆性:
单克隆
- 适应物种:
Human, Mouse
- 保质期:
12 month
- 库存:
50
- 供应商:
LSM bio
- 宿主:
Rabbit
- 应用范围:
ELISA,WB,IHC,IP
- 浓度:
≥95% as determined by SDS-PAGE
- 抗体英文名:
anti-STAT3 antibody,STAT3 antibody
- 抗体名:
anti-STAT3 抗体,STAT3 抗体
- 规格:
100μg
STAT3抗体| STAT3 antibody
货号 PAab08299
蛋白别名 APRF
蛋白介绍
Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)- responsive elements identified in the promoters of various acute- phase protein genes. Activated by IL31 through IL31RA. Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity.
产品描述
anti-STAT3 antibody is a Rabbit Polyclonal antibody againstSTAT3..
建议稀释比例
IHC
Immunohistochemistry of paraffin-embedded human cervical cancer using PAab08299(STAT3 antibody) at dilution of 1:100
Western blot
K-562 cells were subjected to SDS PAGE followed by western blot with PAab08299(STAT3 antibody) at dilution of 1:500(本抗体仅供体外科研用途,不可用于临床诊断!)
武汉维克赛思科技有限公司
地址:武汉东湖新技术开发区东信路光谷创业街10栋
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联系电话:13118103892
Email: info@vecscience.com
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文献和实验【求助】请问用显性负性STAT3和STAT3 mRNA干扰阻断信号差异
semiboat 请问用显性负性STAT3质粒转染阻断IL-6/gp130/STAT3通道和STAT3 mRNA干扰阻断此信号通道有什么差异,在阻断此通道时是否都可以使用,园内各位XDJM有谁知道,非常感谢!! bacchante miRNA干扰比较现代化一些。 显性负作用似乎还要考虑内源蛋白的影响,如果你的启动元件活力不够高,那么你就抑制作用可能就不明显。 另外,似乎不能rescue,也就是说,似乎显性
haohuaiyong 各位战友,最近我在做磷酸化STAT3的检测,用cell signaling公司的磷酸化检测抗体与总STAT3抗体做WB,实验组(bFGF+EGF)与阳性对照的磷酸化水平是有增加的,但总STAT3在实验组是减少的而在对照组是强表达的,我看别人的文献都是将总STAT3作为阳性内参一样,应该是不变化的啊,为什么我做了好多次都是不均一的啊?实在是搞不明白,请做过这方面的战友帮忙!谢谢! dzhzh 你的灌注
maggertu 想请教个问题,做stat3的免疫组化,是选用stat3抗体,还是p-stat3抗体?看到国外大部分文章都做p-stat3,而国内有些文章用stat3抗体,搞不明白 zfyyzz00 stat3抗体只是检测总蛋白,看活性必须要做P-stat3。 在转录水平就应该做EMSA maggertu 谢谢,再请教一下,应该只有磷酸化的stat3才能进行核内转移,那么做stat3抗体
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