- ￥2262
- LSM Bio
- 进口
- PAab04725
- 2025年07月15日
- ELISA,WB,IHC,IP
- E. coli - derived recombinant protein
- Human,Mouse ,Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
lymphocyte-specific protein tyrosine kinase
- 亚型：
IgG
- 形态：
liquid
- 保存条件：
负20摄氏度
- 克隆性：
Polyclonal antibody
- 标记物：
Non-conjugated
- 适应物种：
Human,Mouse ,Rat
- 保质期：
6个月
- 抗原来源：
Rabbit
- 目录编号：
P15248
- 级别：
纯化级别
- 库存：
50
- 供应商：
LSM bio
- 宿主：
E. coli - derived recombinant protein
- 应用范围：
ELISA,WB,IHC,IP
- 浓度：
≥95% as determined by SDS-PAGE
- 靶点：
lymphocyte-specific protein tyrosine kinase
- 抗体英文名：
anti-LCK antibody,LCK antibody
- 抗体名：
anti-LCK 抗体,LCK 抗体
- 规格：
100μg
LCK抗体| LCK antibody
货号 PAab04725
蛋白别名
蛋白介绍
Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T- cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP.
产品描述
anti-LCK antibody is a Rabbit Polyclonal antibody againstLCK..
建议稀释比例
IHC
Immunohistochemistry of paraffin-embedded human lymphoma using PAab04725(LCK antibody) at dilution of 1:100
Western blot
mouse thymus tissue were subjected to SDS PAGE followed by western blot with PAab04725( LCK Antibody) at dilution of 1:1000(本抗体仅供体外科研用途，不可用于临床诊断!) <"">
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文献和实验两只小密封 不好意思，刚开始新的研究方向，涉及到要用基因敲除小鼠模型。有若干不懂的名词求助： 除题目上的问题外，还有类似Egr2 f/f CD4 -/- (右上角的f和-分别代表什么意思呀？有时候还是+/-)。不知道我得问题大家看懂了没有。。。。。实在是汗。在线等答案。谢谢啦！！ laddcat123 Egr2 f/f CD4 -/-: f代表是条件性沉默，利用Cre蛋白可以使其沉默
在免疫反应方面，基于抗原的刺激在体内形成、并与抗原作特异性结合的蛋白质之总称。其化学实质是免疫球蛋白。抗体，按照抗原抗体反应的类型可分别称为沉淀素、凝集素、溶血素、溶菌素、抗毒素、中和抗体等，但这些并不等于有相应的不同抗体存在，一般多只表明抗体反应的多样性而已。抗体在血清中含量最多（体液抗体=humoral antibody）；含有某特定抗体的血清称为抗血或免疫血清。
机体在抗原物质刺激下，由B细胞分化成的浆细胞所产生的、可与相应抗原发生特异性结合反应的免疫球蛋白。因为最初有人用电泳证明血清中抗体活性在γ球蛋白部分，故曾把抗体统称为两种（γ）球蛋白。后来证明，抗体并不都在γ区；而且位于γ区的球蛋白，也不一定都具有抗体活性。1964年，世界卫生组织举行专门会议，将具有抗体活性以及与抗体相关的球蛋白统称为免疫球蛋白（Ig）。如骨髓瘤蛋白，巨球蛋白血症、冷球蛋白血症等患者血清中存在的异常免疫球蛋白以及“正常人”天然存在的免疫
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