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文献和实验Recipe for yeast tryptone medium
. Recipe for DTT Keywords: Zappe; DTT; dithiothreitol Dithiothreitol (DTT) Contributor: Zappe, H Related: many protocols Storage: -20 C Dithiothreitol (DTT) Component stock conc final conc. / 20 ml /1l DTT - 1M 3.09g
Dithiothreitol (DTT) 5 µg/ml* 0.1% silver nitrate* 3% sodium carbonate 37% formaldehyde 2.3M citric acid (* these solutions must be prepared freshly) Staining procedure All volumes are 300 ml for 1.5 x 160 x 160 mm gel (biorad
Purification of Myosin Light Chain Kinase
Solutions 1.1 M Acetic Acid 2.Buffer A Homogenization 10 mM MOPS- 40 ml of 2M stock solution 8 liters 4 mM EDTA- 160ml of 0.2M stock solution 1 mM DTT* - 8ml of 1M stock solution 0.1 mM PMSF* - 14ml of 10mg/ml stock solution 10 μg/ml trypsin
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