The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
1:1000 - 1:3000
IHC
1:50 - 1:200
Western blot analysis of extracts of various cell lines, using VCAM1 Antibody.
Background:
This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene.
Storage:
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.