- 询价
- Signalway Antibody
- C12185
- 2025年09月23日
- WB, IF
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthesized peptide derived from human C/EBP β around the phosphorylation site of T235.
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB, IF
- 靶点：
Uniprot：P17676
- 抗体英文名：
C/EBP β (Phospho-Thr235) Polyclonal Conjugated Antibody
- 规格：
50ul/100ul
计算分子量:36
配方:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
应用详情:WB: 1:50-1:200
IF:1:50-1:200
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文献和实验Polyclonal and Monoclonal Antibodies
or O.N at 4¡C. 4) SECONDARY ANTIBODY a) Wash unbound antibody 4 times with PBS + 0.1% Tween 20. b) Add 100 ul of enzyme linked antibody to all wells. Do the appropriate dilutions in the Elisa buffer. (ex: HRP is 2000X). c
Polyclonal and Monoclonal Antibodies（克隆和克隆抗体）
at 4 C O.N. 3) Spin at 10,000 x g for 10 min. at 4 C to separate the serum. 4) Serum can be stored at -20 C after adding Glycerol to 50%. If monoclonal antibodies are desired, once you have a "good" polyclonal and a reliable screening method
运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 antibody (A) and Total-p38 antibody (B). Cell Based Elisa were performed directly in the 96-well plates using Phospho-p38 and Total-p38 antibodies (C). Figure 3: JNK detection with FACECells were serum-starved for 16 hours
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