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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthesized peptide derived from the C-terminal region of human IL-28.
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Human
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB, IF
- 靶点:
Uniprot:Q8IZJ0
- 抗体英文名:
IL-28 Polyclonal Conjugated Antibody
- 规格:
50ul/100ul
计算分子量:25
配方:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
应用详情:WB: 1:50-1:200
IF:1:50-1:200
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文献和实验Polyclonal and Monoclonal Antibodies
Polyclonal Antibody Immunization of Mice: Injections should be made at intervals of at least two weeks.. We have been using either of the two following adjuvants with success: Freund adjuvant and MPL+TDM adjuvant ( RIBI ImmunoChem
Polyclonal Antibody Production
. If you used xylene, rinse two or three times with ethanol to remove it. Remove the rabbit from the restrainer and place in its cage. Take the collected blood and place at 37 °C for 30 minutes
C 孵育 20 分钟,期间进行连续搅拌,如果是在静置条件下孵育中间进行几次吹打混匀,该孵育时间可适当延长。孵育结束制备得到 CD3/CD28 Streptamer® 混合物; 2. 用培养基(成分:RPMI1640 + 10% 血清 + 50U/ml IL-2 )调整细胞浓度至 5×10^5/ml,将步骤(1)中制备好的 CD3/CD28 Streptamer® complexes 与细胞悬液进行混合,轻轻吹匀后,将细胞加入到细胞培养板中,放置于 37°C 含 5% CO2 细胞培养箱中培养
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