- ￥2062
- LSM Bio
- 进口
- PAab00010
- 2025年07月15日
- ELISA,WB
- E. coli - derived recombinant protein
- Recombinant protein
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 形态：
liquid
- 保存条件：
负20摄氏度
- 克隆性：
Polyclonal antibody
- 标记物：
Non-conjugated
- 适应物种：
Recombinant protein
- 保质期：
6个月
- 抗原来源：
Rabbit
- 目录编号：
Q9GZT9
- 级别：
纯化级别
- 库存：
50
- 供应商：
LSM bio
- 宿主：
E. coli - derived recombinant protein
- 应用范围：
ELISA,WB
- 浓度：
≥95% as determined by SDS-PAGE
- 靶点：
6*his,His tag
- 抗体英文名：
anti-6*his,His tag antibody,6*his,His tag antibody
- 抗体名：
anti-6*his,His tag 抗体,6*his,His tag 抗体
- 规格：
100μg
6*his,His tag抗体| 6*his,His tag antibody
货号 PAab00010
蛋白别名 6*His antibody, 6*His antibody, His Tag antibody, 6histag antibody, 6XHis antibody, HHHHHH antibody, HIS antibody, His Tag antibody, HISTIDINE TAGGED
蛋白介绍
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.
产品描述
anti-6*his,His tag antibody is a Rabbit Polyclonal antibody against6*his,His tag..
建议稀释比例
WB : 1:1000-1:5000
IHC
Western blot
Western blot of 6*His-tagged fusion protein with PAab00010(anti-6*HIS tag) at dilution of 1:2000.(本抗体仅供体外科研用途，不可用于临床诊断!)
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文献和实验基因工程抗体(genetically engineered antibody)
。由于受细胞内蛋白质加工、修饰能力的限制，大肠 杆菌不适合表达完整的抗体分子。在表达产物末端加入一个标签序列（ｔａｇ ｓｅｑｕｅｎｃｅ）是一种通用的方法，由此可方便地用ＥＬＩＳＡ 等常规 方法对表达产物定量检测，以及用亲和层析方法予以纯化。标签位点通常置于产物的Ｃ －末端，这样既不 影响抗体的活性，又不影响标签的特异性。常用的标签是由６ 个组氨酸与Ｎｉ２ ＋、Ｚｎ２ ＋及Ｃｏ２ ＋等阳离子组 成的组氨酸串。 酵母（ｙｅａｓｔ）是一类单细胞真核生物，不产生毒素，遗传背景清晰。在表达抗体时具有
【共享】如何检测GFP、GFP、YFP、EYFP、CFP抗体？
对照，2道为1:1000倍稀释我们先看一下EarthOx的单克隆GFP抗体——Anti-GFP Tag Monoclonal Antibody [GF28R]（货号#E022030），其免疫原是KLH偶联的水母GFP蛋白的N末端多肽，由于EGFP、YFP、EYFP、CFP得N末端序列与GFP几乎完全一致，因此从免疫原上来说，GFP与EGFP、YFP、EYFP、CFP没有区别。更重要的是，EarthOx对于该单克隆抗体还进行了明确的验证试验，试验也表明该Anti-GFP Tag Monoclonal
Purification and Analysis of Strep-tagged Antibody-Fragments
The Strep-tag is a nine-residue minimal peptide sequence with intrinsic affinity towards streptavidin. This peptide – or its improved version Strep-tag II – can be fused to recombinant antibody fragments and other proteins in various fashions
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