VSV-FL+(2) VSV Plasmid Express

Preparation and Quantification of Pseudotyped Retroviral Vector

and biochemical inactivation. A cell line producing VSV-G pseudotyped MuLV vector can be established by transfecting 293T cells expressing Gag, Pol, and VSV-G (293 GPG cell line) with a retroviral vector plasmid. Transduction potency of the resulting VSV-G

Production of Lentiviral Vector Supernatants and Transduction of Cellular Targets

particles. At top are the three plasmids pHIVPV, pHIV-TV, and the VSV G expression plasmid. A tri-transfection of 293T cells is performed by the calcium-phosphate technique. After 2–3 d, the supernatant is harvested (pseudotyped particles shown), and used

Creation and Use of Infectious Virus Vector

for lenti: 1 μg packaging (pHR’8.2deltaR) at a 8:1 ratio with the envelope plasmid (pCMV-VSV-G). for MuLV: the packaging plasmid (pUMVC3) at a 8:1 ratio with the envelope plasmid (pCVM-VSV-G)-a total of 1ug. DME