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文献和实验by the presence of l -malate in the growth medium. We used the regulative region comprising the two-component system located upstream the PSHAb0363 gene to construct an inducible expression vector – named pUCRP – under the control of l -malate
Recombinant Protein Production in Antarctic Gram-Negative Bacteria
in the construction of a shuttle vector, able to replicate either in E. coli or in several cold-adapted hosts (clone Q ). Since the conversion of a cloning system into an expression vector requires the insertion of transcription and translation
Plasmid Artificial Modification: A Novel Method for Efficient DNA Transfer into Bacteria
host). Propagation of a shuttle vector from the PAM host to the target bacterium ensures that the plasmid will be modified such that it is protected from restriction endonuclease digestion in the target bacterium. The result will be a higher
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