Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human BID
Calculated molecular weight
11kDa/14kDa/21kDa/26kDa
Observed molecular weight
22kDa
Swiss Prot
P55957
Gene ID (NCBI)
637
Alternative Names
BID; FP497; BH3-interacting domain death agonist
Application Notes:
WB
1:500 - 1:2000
IF
1:50 - 1:200
Western blot analysis of extracts of HL-60 cells, using BID Antibody.
Immunofluorescence analysis of C6 cells using BID antibody
Immunofluorescence analysis of NIH/3T3 cells using BID antibody
Immunofluorescence analysis of U2OS cells using BID antibody
Background:
This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined.
Storage:
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.