Anti-Yin and yang 1

The Concept of Multiple-Target Anti-miRNA Antisense Oligonucleotide Technology

as an miRNA therapy for human disease such as cancer and cardiac disorders. This technology was developed by my research laboratory in collaboration with Yang’s group (Lu et al., Nucleic Acids Res 37:e24–e33, 2009), and it is similar but distinct

9.23 上交复旦Science子刊揭示靶向YY1有望治疗糖尿病肾病

① 上交复旦Science 子刊揭示靶向 YY1 有望治疗糖尿病肾病转化生长因子β1（TGFβ1，由基因 TGFB1 编码）已被确定为糖尿病肾病产生的主要致病因子。然而，当前的拮抗 TGFβ1 的策略未能在临床试验中表现出有益的治疗结果。在一项新的研究中，来自中国上海交通大学和复旦大学等研究机构的研究人员为了鉴定出一种不同的治疗方法，设计出一种基于质谱的 DNA-蛋白相互作用筛选方法，以便发现与 TGFβ1 启动子结合的转录阻遏物。他们鉴定出一种称为阴阳 1（Yin Yang 1, YY

Map-Based Cloning（图位克隆技术）

downstream of the stop codon, was amplified from genomic DNA and cloned into a binary vector pCAMBIA 1301, then transformed into fon4-1 by Agrobacterium-mediated transformation (Hiei et al., 1994). From: Huangwei Chu, Qian Qian, Wanqi Liang, Changsong Yin