The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
1:1000 - 1:2000
IF
1:50 - 1:200
IHC
1:50-1:500
Western blot analysis of extracts of Jurkat cells, using PARP1 antibody.
Western blot analysis of extracts of mouse testis, using PARP1 antibody.
Immunofluorescence analysis of NIH/3T3 cells using PARP1 antibody.
Immunofluorescence analysis of U2OS cells using PARP1 antibody.
Background:
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
Storage:
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.|Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.