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Roche货号11097113001与10269638001

胶原酶/中性蛋白酶19121878899上海睿安生物
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  • ¥2500.13
  • Roche
  • 11097113001与10269638001
  • Made in Germany
  • 2026年04月08日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 英文名

      Collagenase

    • 保质期

      1年

    • 保存条件

      2-8°C

    • 库存

      100⁺瓶

    • 供应商

      上海睿安生物19121878899

    • 规格

      100mg/瓶

    Roche货号10269638001胶原酶/中性蛋白酶19121878899上海睿安生物

     

    Roche货号11097113001胶原酶/中性蛋白酶19121878899上海睿安生物

    胶原酶/中性蛋白酶®

    lyophilized,non-sterile,optimum pH 7.0-8.0

    别名:胶原蛋白酶

    说明

    一般描述

    分散酶是一种中性、快速反应、温和的蛋白酶,能将表皮从真皮中完整分离。它还能将培养的上皮细胞从基质中完整分离出来。其可分裂基底膜区,使上皮细胞保持完整。胶原酶是一种分解三螺旋蛋白胶原蛋白的蛋白酶。组织胶原酶分三种类型,它们属于基质金属蛋白酶(MMP)家族。从溶组织性梭状芽孢杆菌中提取的胶原酶具有很强的活性,因为它可在低至4-10℃的温度下,从两端消化胶原蛋白。

    特异性:胶原酶降解天然胶原蛋白。中性蛋白酶® 是一种非特异性的金属蛋白酶。

    应用:胶原酶/中性蛋白酶® 用于从多种不同的组织和器官中制备细胞。

    组分:EC 3.4.24.4 & 3.4.24.3

    单位定义:一个单位是指,在1分钟内在反应条件下释放出1 μmol酪氨酸对应的Folin-阳性氨基酸和多肽所需的酶活性。

    制备说明

    激活剂:Ca2+

    工作浓度:约为1mg/ml(胶原酶:0.1U/ml,中性蛋白酶:0.8U/ml)

    酶活性:
    胶原酶:0.1Wünsch U/mg冻干粉(+25°C,4-苯基-偶氮苄基-氧基羰基-Pro-Leu-Gly-Pro-D-Arg作为底物)
    中性蛋白酶®:0.8Wünsch U/mg冻干粉(+37°C,酪蛋白为底物,pH 7.5)
    污染蛋白水解活性:<0.1U/mg 冻干粉(血红蛋白作为底物)

    工作溶液:工作溶液制备将酶溶于水中,使其终浓度为100mg/ml。用PBS(不含Ca2+/Mg2+)稀释至终浓度为1mg/ml,并采用无菌0.2μm过滤膜过滤。
    吴军溶液的酶浓度:胶原酶,0.1U/ml PBS;中性蛋白酶®,0.8U/ml PBS。

    抑制剂:
    胶原酶抑制剂:EDTA,EGTA
    血清不能抑制胶原酶/中性蛋白酶。

    保存条件(工作溶液):-15至-25°C
    复溶的储备溶液在-15至-25°C下稳定。
    注:适当分装保存并避免反复冻融!

    重悬

    复溶于水中(100mg/ml)后续稀释采用PBS(磷酸缓冲盐)(不含Ca2+/Mg2+)。

    储存及稳定性:干燥储存

    其他说明:仅用于生命科学研究。不可用于诊断。胶原酶来源于 溶藻弧菌, 中性蛋白酶® 来源于 多粘芽孢杆菌。

    同行评审论文(部分文献)

    Multiple Antenatal Dexamethasone Treatment Alters Brain Vessel Differentiation in Newborn Mouse Pups.
    Winfried Neuhaus et al.
    PloS one, 10(8), e0136221-e0136221 (2015-08-15)
    Antenatal steroid treatment decreases morbidity and mortality in premature infants through the maturation of lung tissue, which enables sufficient breathing performance. However, clinical and animal studies have shown that repeated doses of glucocorticoids such as dexamethasone and betamethasone lead to
    Endometrial organoids derived from Mayer-Rokitansky-Küster-Hauser syndrome patients provide insights into disease-causing pathways.
    Sara Y Brucker et al.
    Disease models & mechanisms, 15(5) (2022-04-09)
    The uterus is responsible for the nourishment and mechanical protection of the developing embryo and fetus and is an essential part in mammalian reproduction. Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome is characterized by agenesis of the uterus and upper part of the vagina
    Novel leukocyte-depleted platelet-rich plasma-based skin equivalent as an in vitro model of chronic wounds: a preliminary study.
    Elisa Seria et al.
    BMC molecular and cell biology, 22(1), 28-28 (2021-05-12)
    Chronic leg ulcerations are associated with Haemoglobin disorders, Type2 Diabetes Mellitus, and long-term venous insufficiency, where poor perfusion and altered metabolism develop into a chronic inflammation that impairs wound closure. Skin equivalent organotypic cultures can be engineered in vitro to
    A detailed protocol for a rapid analysis of testicular cell populations using flow cytometry.
    E Rotgers et al.
    Andrology, 3(5), 947-955 (2015-08-11)
    Accurate analysis and quantification of different testicular cell populations are of central importance in studies of male reproductive biology. The traditional histomorphometric and immunohistochemical methods remain the gold standard in studying the complex dynamics of the testicular tissue. Through past
    Electrical coupling between Aplysia bag cell neurons: characterization and role in synchronous firing.
    Zahra Dargaei et al.
    Journal of neurophysiology, 112(11), 2680-2696 (2014-09-05)
    In neuroendocrine cells, hormone release often requires a collective burst of action potentials synchronized by gap junctions. This is the case for the electrically coupled bag cell neurons in the reproductive system of the marine snail, Aplysia californica. These neuroendocrine

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    Roche货号10269638001胶原酶/中性蛋白酶19121878899上海睿安生物

     

    Roche货号11097113001胶原酶/中性蛋白酶19121878899上海睿安生物

    胶原酶/中性蛋白酶®

    lyophilized,non-sterile,optimum pH 7.0-8.0

    别名:胶原蛋白酶

    说明

    一般描述

    分散酶是一种中性、快速反应、温和的蛋白酶,能将表皮从真皮中完整分离。它还能将培养的上皮细胞从基质中完整分离出来。其可分裂基底膜区,使上皮细胞保持完整。胶原酶是一种分解三螺旋蛋白胶原蛋白的蛋白酶。组织胶原酶分三种类型,它们属于基质金属蛋白酶(MMP)家族。从溶组织性梭状芽孢杆菌中提取的胶原酶具有很强的活性,因为它可在低至4-10℃的温度下,从两端消化胶原蛋白。

    特异性:胶原酶降解天然胶原蛋白。中性蛋白酶® 是一种非特异性的金属蛋白酶。

    应用:胶原酶/中性蛋白酶® 用于从多种不同的组织和器官中制备细胞。

    组分:EC 3.4.24.4 & 3.4.24.3

    单位定义:一个单位是指,在1分钟内在反应条件下释放出1 μmol酪氨酸对应的Folin-阳性氨基酸和多肽所需的酶活性。

    制备说明

    激活剂:Ca2+

    工作浓度:约为1mg/ml(胶原酶:0.1U/ml,中性蛋白酶:0.8U/ml)

    酶活性:
    胶原酶:0.1Wünsch U/mg冻干粉(+25°C,4-苯基-偶氮苄基-氧基羰基-Pro-Leu-Gly-Pro-D-Arg作为底物)
    中性蛋白酶®:0.8Wünsch U/mg冻干粉(+37°C,酪蛋白为底物,pH 7.5)
    污染蛋白水解活性:<0.1U/mg 冻干粉(血红蛋白作为底物)

    工作溶液:工作溶液制备将酶溶于水中,使其终浓度为100mg/ml。用PBS(不含Ca2+/Mg2+)稀释至终浓度为1mg/ml,并采用无菌0.2μm过滤膜过滤。
    吴军溶液的酶浓度:胶原酶,0.1U/ml PBS;中性蛋白酶®,0.8U/ml PBS。

    抑制剂:
    胶原酶抑制剂:EDTA,EGTA
    血清不能抑制胶原酶/中性蛋白酶。

    保存条件(工作溶液):-15至-25°C
    复溶的储备溶液在-15至-25°C下稳定。
    注:适当分装保存并避免反复冻融!

    重悬

    复溶于水中(100mg/ml)后续稀释采用PBS(磷酸缓冲盐)(不含Ca2+/Mg2+)。

    储存及稳定性:干燥储存

    其他说明:仅用于生命科学研究。不可用于诊断。胶原酶来源于 溶藻弧菌, 中性蛋白酶® 来源于 多粘芽孢杆菌。

    同行评审论文(部分文献)

    Multiple Antenatal Dexamethasone Treatment Alters Brain Vessel Differentiation in Newborn Mouse Pups.
    Winfried Neuhaus et al.
    PloS one, 10(8), e0136221-e0136221 (2015-08-15)
    Antenatal steroid treatment decreases morbidity and mortality in premature infants through the maturation of lung tissue, which enables sufficient breathing performance. However, clinical and animal studies have shown that repeated doses of glucocorticoids such as dexamethasone and betamethasone lead to
    Endometrial organoids derived from Mayer-Rokitansky-Küster-Hauser syndrome patients provide insights into disease-causing pathways.
    Sara Y Brucker et al.
    Disease models & mechanisms, 15(5) (2022-04-09)
    The uterus is responsible for the nourishment and mechanical protection of the developing embryo and fetus and is an essential part in mammalian reproduction. Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome is characterized by agenesis of the uterus and upper part of the vagina
    Novel leukocyte-depleted platelet-rich plasma-based skin equivalent as an in vitro model of chronic wounds: a preliminary study.
    Elisa Seria et al.
    BMC molecular and cell biology, 22(1), 28-28 (2021-05-12)
    Chronic leg ulcerations are associated with Haemoglobin disorders, Type2 Diabetes Mellitus, and long-term venous insufficiency, where poor perfusion and altered metabolism develop into a chronic inflammation that impairs wound closure. Skin equivalent organotypic cultures can be engineered in vitro to
    A detailed protocol for a rapid analysis of testicular cell populations using flow cytometry.
    E Rotgers et al.
    Andrology, 3(5), 947-955 (2015-08-11)
    Accurate analysis and quantification of different testicular cell populations are of central importance in studies of male reproductive biology. The traditional histomorphometric and immunohistochemical methods remain the gold standard in studying the complex dynamics of the testicular tissue. Through past
    Electrical coupling between Aplysia bag cell neurons: characterization and role in synchronous firing.
    Zahra Dargaei et al.
    Journal of neurophysiology, 112(11), 2680-2696 (2014-09-05)
    In neuroendocrine cells, hormone release often requires a collective burst of action potentials synchronized by gap junctions. This is the case for the electrically coupled bag cell neurons in the reproductive system of the marine snail, Aplysia californica. These neuroendocrine

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    Roche货号11097113001与10269638001胶原酶/中性蛋白酶19121878899上海睿安生物
    ¥2500.13