重组人ME2(带His标签)价格

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产品名称：重组人ME2(带His标签)
英文名称：Recombinant Human Malic Enzyme 2, His
产品货号：GS4629
产品规格：5μg

分子量：Approximately 64.4kDa,a single non-glycosylated polypeptide chain containing 573 amino acids with 6×His at C-terminus.
储存条件：冷冻（-20℃）

概述

NAD-dependent malic enzyme(ME2),mitochondrial is a protein that in humans is encoded by the ME2 gene.This gene encodes a mitochondrial NAD-dependent malic enzyme,a homotetrameric protein,which catalyzes the oxidative decarboxylation of malate to pyruvate.Three different isoforms of ME are known to be in mammalian tissues:a strictly cytosolic NADP+-dependent enzyme,an NADP+-dependent mitochondriail isoform,and a mitochondrial isoenzyme that is able to use both NAD+ and NADP+ but is more effective with NAD+.The mammalian isoforms size is about 62-64 kDa.A native size of 240,000 Da proposes a tetrameric structure for the active enzyme.

使用说明

This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.

技术规格
 

外观	Sterile Filtered White lyophilized(freeze-dried)powder.
纯度	＞95%by SDS-PAGE and HPLC analyses.
来源	Escherichia coli.
生物活性	Malic Enzyme activity was assayed spectrophotometrically at 340nmas described in Mandela and Sauer(1975).The standard reaction mixture contained 50mMTris-HCl,3 mM MnCl2,5 mM malate,0.12 mM NADP+,2.5 mM fumarate.Assay was performed in a Beckman spectrophotometer.The Km value is 1.5 ± 0.6 mM.
成分	Lyophilized from a 0.2μmfiltered concentrated solution in PBS,pH 7.4.
内毒素	Less than 1 EU/μg of rHuME2,His as determined by LAL method.
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions.
氨基酸序列	MLHIKEKGKP LMLNPRTNKG MAFTLQERQM LGLQGLLPPK IETQDIQALR FHRNLKKMTS PLEKYIYIMG IQERNEKLFY RILQDDIESL MPIVYTPTVG LACSQYGHIF RRPKGLFISI SDRGHVRSIV DNWPENHVKA VVVTDGERIL GLGDLGVYGM GIPVGKLCLY TACAGIRPDR CLPVCIDVGT DNIALLKDPF YMGLYQKRDR TQQYDDLIDE FMKAITDRYG RN

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