重组小鼠LIF北京厂家

特别提示：包括重组小鼠LIF在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：重组小鼠LIF
英文名称：Recombinant Murine Leukemia inhibitory Factor
产品货号：GS4850
产品规格：5μg

分子量：Approximately 19.9kDa,a single non-glycosylated polypeptide chain containing 180 amino acids.
储存条件：冷冻（-20℃）

概述

Leukemia inhibitory factor(LIF)is a member of Interleukin 6 family.This protein is mainly expressed in the trophectoderm of the developing embryo,with its receptor LIFR expressed throughout the inner cell mass.LIF has the capacity to induce terminal differentiation in leukemic cells.Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells,the induction of neuronal cell differentiation,and the stimulation of acute-phase protein synthesis in hepatocytes.LIF is used in mouse embryonic stem cell culture,because that removal of LIF pushes stem cells toward differentiation,but they retain their proliferative potential or pluripotency.It is also used in phase II clinical trial,which can assist embryo implantation in women who have failed to become pregnant despite assisted reproductive technologies(ART).Mature mouse LIF shares 78%a.a.sequence identity with Human LIF.

使用说明

This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.

技术规格
 

外观	Sterile Filtered White lyophilized(freeze-dried)powder.
纯度	＞98%by SDS-PAGE and HPLC analyses.
来源	Escherichia coli.
生物活性	Fully biologically active when compared to standard.The specific activity is determined by inducing differentiation of murine M1 myeloid leukemic cells.The minimum detectable concentration of rMuLIF in this assay is 0.01ng/ml.The specific activity of>1.0×108 IU/mg,where 50 units is defined as the amount of rMuLIF required to induce differentiation in 50%of the M1 colonies in 1mlagar cultures.
成分	Lyophilized from a 0.2μmfiltered concentrated solution in 20mMPB,pH 7.4,with 0.02%TWEEN 20.
内毒素	Less than 1 EU/μg of rMuLIF as determined by LAL method.
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions.
氨基酸序列	SPLPITPVNA TCAIRHPCHG NLMNQIKNQL AQLNGSANAL FISYYTAQGE PFPNNVEKLC APNMTDFPSF HGNGTEKTKL VELYRMVAYL SASLTNITRD QKVLNPTAVS LQVKLNATID VMRGLLSNVL CRLCNKYRVG HVDVPPVPDH SDKEAFQRKK LGCQLLGTYK QVISVVVQAF

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