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SK-UT-1 |
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G Trempe, LJ Old |
Biosafety Level: |
1 |
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frozen |
Medium & Serum: |
See Propagation |
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adherent |
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Homo sapiens |
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epithelial
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Organ: uterus Tumor Stage: grade III Disease: mesodermal tumor (mixed); consistent with leiomyosarcoma |
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
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The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439. |
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Yes |
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Blood Type B; Rh+ |
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Amelogenin: X CSF1PO: 10,11 D13S317: 13 D16S539: 13,14 D5S818: 10,11 D7S820: 9,10 THO1: 7 TPOX: 8 vWA: 15,16 |
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(P8) hypodiploid to hyperdiploid |
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AK-1, 1 ES-D, 1 G6PD, B Me-2, 1-2 PGM1, 1 PGM3, 1 |
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75 years |
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female |
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Caucasian |
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ATCC complete growth medium: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10% Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
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Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:12 is recommended. Medium Renewal: Twice per week Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
- Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
- Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting
- Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37C.
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Culture medium, 95%; DMSO, 5% |
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21869: . Human tumor cells in vitro. New York: Plenum Press; 1975. 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 |