Organ: brain Disease: neuroblastoma Derived from metastatic site: bone marrow Cell Type: neuroblast;
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SK-N-AS cells synthesize Insulin-like growth factor II (IFG-II) and IGF-II RNA and possess type I IGF receptors [Pubmed ID: 2547840]. Retinoic acid partially inhibits proliferation, and cells fail to differentiate [Pubmed ID: 1751405]. The cells exhibit low or no MDR1 expression.
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
O.1 mM Non-Essential Amino Acids (NEAA)
fetal bovine serum to a final concentration of 10%
Temperature: 37.0°C
Subculturing:
Protocol: Remove spent medium, add fresh 0.25% trypsin, 0.53 MM EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended Medium Renewal: Twice per week
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020
References:
22290: Sugimoto T, et al. Determination of cell surface membrane antigens common to both human neuroblastoma and leukemia-lymphoma cell lines by a panel of 38 monoclonal antibodies. J. Natl. Cancer Inst. 73: 51-57, 1984. PubMed: 6610792 22439: Iavarone A, et al. Uptake and storage of m-iodobenzylguanidine are frequent neuronal functions of human neuroblastoma cell lines. Cancer Res. 53: 304-309, 1993. PubMed: 8417824 22444: Gaetano C, et al. Retinoic acid negatively regulates p34cdc2 expression during human neuroblastoma differentiation. Cell Growth Differ. 2: 487-493, 1991. PubMed: 1751405 22500: El-Badry OM, et al. Autonomous growth of a human neuroblastoma cell line is mediated by insulin-like growth factor II. J. Clin. Invest. 84: 829-839, 1989. PubMed: 2547840