Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology:
lymphoblast
Source:
Cell Type: hybridoma: B lymphocyte;
Cellular Products:
immunoglobulin; monoclonal antibody; against Lgp100a (a 100000 dalton glycoprotein that is expressed on mouse T and B cells)
Permits/Forms:
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Comments:
Animals were immunized with mouse splenic or thymic lymphocytes. Spleen cells were fused with NS-1 myeloma cells. Tested and found negative for ectromelia virus (mousepox).
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.