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Restrictions:
This material is not available for international distribution due to the lack of historical data to support the issuance of a U.S. Fish and Wildlife Service Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES) export permit
Virus Resistance:
poliovirus 1
Comments:
Growth is inhibited at alkaline pH. Medium should be adjusted to neutrality after plating.
ATCC complete growth medium: Basal medium (Eagle) in Hanks' BSS, 90%; fetal bovine serum, 10%
Subculturing:
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended Medium Renewal: Every 10 to 14 days Remove medium, add fresh 0.25% trypsin for 2 to 3 minutes, remove trypsin and allow the culture to stand for 5 to 10 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks.
References:
877: Clark HF, Karzon DT. Acquired tolerance to elevated temperatures in a poikilothermic cell line (terrapene heart, TH-1). Exp. Cell Res. 48: 269-275, 1967. PubMed: 6082311 878: Clark HF, Karzon DT. Terrapene heart (TH-1), a continuous cell line from the heart of the box turtle Terrapene carolina. Exp. Cell Res. 48: 263-268, 1967. PubMed: 4295486 57990: Huang CC, Clark HF. Chromosome changes in cell lines of the box turtle (Terrapene carolina) grown at two different temperatures. Can. J. Genet. Cytol. 9: 449-461, 1967. PubMed: 6082587