immunoglobulin; monoclonal antibody; against a human melanoma cell line (M-11 antigen system)
Permits/Forms:
In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:
The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.
Comments:
Animals were immunized with the SK-MEL-19 human melanoma cell line. The antibody reacts with and precipitates a 45000 dalton glycoprotein from extracts of SK-MEL-19 cells. The antibody also shows a wide range of reactivity against tumor tissues, tumor cell lines and normal tissues. The antibody reacts with the M-11 antigen system.
ATCC complete growth medium: RPMI 1640 medium with non-essential amino acids, 90%; fetal bovine serum, 10%
Subculturing:
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation:
culture medium, 90%; DMSO, 10%
References:
1654: Houghton AN, et al. Surface antigens of melanocytes and melanomas. Markers of melanocyte differentiation and melanoma subsets. J. Exp. Med. 156: 1755-1766, 1982. PubMed: 7175440 1657: Real FX, et al. Surface antigens of melanomas and melanocytes defined by mouse monoclonal antibodies: Specificity analysis and comparison of antigen expression in cultured cells and tissues. Cancer Res. 45: 4401-4411, 1985. PubMed: 4028024 1658: Dracopoli NC, et al. Three human cell surface antigen systems determined by genes on chromosome. Somatic Cell Mol. Genet. 10: 475-481, 1984. PubMed: 6206575 3891: Cairncross JG, et al. Monoclonal antibodies against melanocytes. US Patent 4,806,628 dated Feb 21 1989 3892: Oettgen HF, et al. Monoclonal antibodies to cell surface antigens of human malignant melanoma. US Patent 4,808,704 dated Feb 28 1989