Organ: spleen Strain: BALB/c Cell Type: hybridoma:lymphoblast B lymphocyte;
Cellular Products:
immunoglobulin; monoclonal antibody; against factors VII and VIIa (VII/VIIa)
Permits/Forms:
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Age:
adult
Gender:
female
Comments:
Animals were immunized with purified human factor VII (factor VII) isolated from pooled human plasma. Spleen cells were fused with Sp2/0 mouse myeloma cells. The 12D10 monoclonal antibody reacts specifically with both factor VII and factor VIIa antigen. [57782] It is specific to amino acids 195-208 on the factor VII/VIIa molecule. [57782]
ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and RPMI 1640 medium with 3 mM L-glutamine and 4.5 g/L glucose, 90%; fetal bovine serum, 10% Temperature: 37.0°C
Subculturing:
Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 1 X 10 exp5 viable cells/ml. Maintain cultures at cell concentrations between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. Medium Renewal: Two to three times weekly
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020
References:
57782: Soule HR, Brunck TK. Hypridoma and monoclonal antibody which inhibits blood coagulation tissue factor/factor VIIa complex. US Patent 5,506,134 dated Apr 9 1996 57783: Biemond BJ, et al. Complete inhibition of endotoxin-induced coagulation activation in chimpanzees with a monoclonal Fab fragment against factor VII/VIIa. Thromb. Haemostasis 73: 223-230, 1995. PubMed: 7792734