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MCF-10-2A |
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Michigan Cancer Foundation |
Biosafety Level: |
1 |
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frozen |
Medium & Serum: |
See Propagation |
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adherent |
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Homo sapiens |
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epithelial
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Organ: mammary gland; breast Disease: fibrocystic disease |
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cytokeratin 19 |
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
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transfection host |
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epidermal growth factor (EGF); insulin; glucocorticoid |
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No |
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Amelogenin: X CSF1PO: 10,12 D13S317: 9 D16S539: 11,12 D5S818: 10,13 D7S820: 10,11 THO1: 8 TPOX: 9,11 vWA: 15,17 |
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modal number = 40 to 43; XX; five marker chromosomes; double minutes |
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36 years |
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female |
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ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium supplemented with 20 ng/ml epidermal growth factor, 100 ng/ml cholera toxin, 0.01 mg/ml insulin and 500 ng/ml hydrocortisone, 95%; horse serum, 5% Temperature: 37.0°C |
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Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Every 2 to 3 days Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
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culture medium 95%; DMSO, 5% |
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derived from same individual:ATCC CRL-10317 derived from same individual:ATCC CRL-10318 derived from same individual:ATCC CRL-10780 |
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21968: Soule H, McGrath CM. Immortal human mammary epithelial cell lines. US Patent 5,026,637 dated Jun 25 1991 22025: Pauley RJ, et al. Immortal human mammary epithelial cell sublines. US Patent 5,206,165 dated Apr 27 1993 22043: Pauley RJ, et al. Immortal human mammary epithelial cell sublines. US Patent 5,238,840 dated Aug 24 1993 22248: Soule HD, McGrath CM. A simplified method for passage and long-term growth of human mammary epithelial cells. In Vitro Cell. Dev. Biol. 22: 6-12, 1986. PubMed: 2418007 23084: Soule HD, et al. Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6075-6086, 1990. PubMed: 1975513 23085: Tait L, et al. Ultrastructural and immunocytochemical characterization of an immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6087-6094, 1990. PubMed: 1697506 |