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Cytogenetic Analysis:
normal diploid female
Isoenzymes:
G6PD, B
Age:
infant
Gender:
female
Ethnicity:
Caucasian
Comments:
From patient with citrullinemia. Deficient in citrulline metabolism.
ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Subculturing:
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended Medium Renewal: Add fresh medium at the time of subculture Remove medium, add fresh 0.25% trypsin solution, remove trypsin and let the culture stand for 5 to 10 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks.
References:
22175: . . Proc. Natl. Acad. Sci. USA 57: 829, 1967.