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- 详细信息
- 文献和实验
- 技术资料
- 库存:
276
- 英文名:
Succinimidyl-6-(biotinamido)hexanoate
- 保质期:
长期
- 供应商:
北京百奥莱博科技有限公司
- 保存条件:
冷冻(-20℃)
- 规格:
50mg
特别提示:包括NHS-LC-Biotin在内,本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!
产品名称:NHS-LC-Biotin
英文名称:Succinimidyl-6-(biotinamido)hexanoate
产品货号:GS4322
产品规格:50mg
分子量:454.5
储存条件:冷冻(-20℃)
概述
NHS-Biotin Reagents enable simple and efficient biotin labeling of antibodies,proteins and any other primary
amine-containing macromolecules in solution.Differing only in spacer arm lengths,the three reagents offer researchers the
possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor.
应用
NHS LC Biotin Reagents are not directly water soluble and must be
dissolved in organic solvents such as DMSO or DMF before addition to aqueous solutions at the final concentration for the
labeling reaction.
组份
50mg/bottle
技术规格
| 分子量 | 454.54 |
| 间隔臂 | 22.4 |
| Net Mass Added | 339.16 |
| 纯度 | >95%on TLC |
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文献和实验8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
were prepared by labeling BSA with Cy3 and Cy5 monofunctional reactive dyes (Amersham Pharmacia Biotech). Cy5-BSA-biotin was prepared by labeling Cy5-BSA with Sulfo-NHS-LC-Biotin (Pierce). Cy3-BSA-maleimide was prepared by labeling Cy3-BSA with Sulfo-GMBS
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