①RLC = relative luminescence capacity:Total time-integrated emission of aequorin in saturating Ca2+relative to native aequorin = 1.0.
②Ratio of the luminescence of aequorin reconstituted with coelenterazine analog relative to native aequorin at 100 nM Ca2+.
③Half-Rise Time:The half-rise time is the time for the luminescence signal to reach 50%of the maximum after addition of 1mMCa2+to a standard of aequorin reconstituted with the coelenterazine analog of interest.