重组人TNF-α,Variant北京厂家

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产品名称：重组人TNF-α,Variant
英文名称：Recombinant Human Tumor Necrosis Factor-alpha, Variant
产品货号：GS4495
产品规格：10μg

分子量：Approximately 16.9kDa,a single non-glycosylated polypeptide chain containing 151 amino acids.Compared with the wild-type,rHuTNF-α Variant has an amino acid sequence(a.a.)deletion from a.a.1-7,and the following a.a.substitutes Arg8,Lys9,Arg10 an
储存条件：冷冻（-20℃）

概述

The clinical use of the potent antitumor activity of TNF-α has been limited by the proinflammatory side effects including fever,dose-limiting hypotension,hepatotoxicity,intravascular thrombosis,and hemorrhage.Designing clinically applicable TNF-αmutants with low systemic toxicity has been an intense pharmacological interest.Human TNF-α,which binds to the murine TNF-R55 but not to the murine TNF-R75,exhibits retained antitumor activity and reduced systemic toxicity in mice compared with murine TNF-α,which binds to both murine TNF receptors.Based on these results,many TNF-αmutants that selectively bind to TNF-R55 have been designed.These mutants displayed cytotoxic activities on tumor cell lines in vitro,and exhibited lower systemic toxicity in vivo.

使用说明

This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.

技术规格
 

外观	Sterile Filtered White lyophilized(freeze-dried)powder.
纯度	＞98%by SDS-PAGE and HPLC analyses.
来源	Escherichia coli.
生物活性	Fully biologically active when compared to standard.The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.01ng/ml,corresponding to a specific activity of>1.0×108 IU/mg in the presence of actinomycin D.
成分	Lyophilized from a 0.2μmfiltered concentrated solution in PBS,pH 7.0.
内毒素	Less than 1 EU/μg of rHu TNF-α,Variant as determined by LAL method.
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20?C.Further dilutions should be made in appropriate buffered solutions.
氨基酸序列	MRKRKPVAHV VANPQAEGQL QWLNRRANAL LANGVELRDN QLVVPSEGLY LIYSQVLFKG QGCPSTHVLL THTISRIAVS YQTKVNLLSA IKSPCQRETP EGAEAKPWYE PIYLGGVFQL EKGDRLSAEI NRPDYLDFAE SGQVYFGIIA F

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