Human KGF-1 also known as Fibroblast growth factor 7(FGF-7),is encoded by the FGF7 gene.KGF-1 only binds to the b splice form of the tyrosine kinase receptor,FGFR2b/KGFR.Affinity between KGF-1 and its receptor can be increased by heparin or heparin sulfate proteoglycan.FGF-10,also called keratinocyte growth factor 2(KGF-2),shares 51%amino acid sequence identity and similar function to KGF-1,but uses an additional receptor,FGFR2c.KGF-1 plays an important role in the regulation of embryonic development,cell proliferation and cell differentiation.KGF-1 actives on keratinocytes,and exhibits mitogenic activity for epidermal cells,but essentially no activity for fibroblasts.KGF-1 has species crossactive,human KGF-1 shares 96%and 92%amino acid sequence identity with murine and rat FGF-7,respectively.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
外观
Sterile Filtered White lyophilized(freeze-dried)powder.
纯度
>96%by SDS-PAGE and HPLC analyses.
来源
Escherichia coli.
生物活性
Fully biologically active when compared to standard.The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 10ng/ml,corresponding to a specific activity of>1.0×105 IU/mg.
成分
Lyophilized from a 0.2μmfiltered solution in 20mMPB,0.5MNaCl,pH 8.0.
内毒素
Less than 1 EU/μg of rHuKGF-1/FGF-7 as determined by LAL method.
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions.