2,3-Dichlorophenylhydrazine Hy

Synthesis of Peptidomimetic Conjugates of Cyclic Nucleoside Phosphonates

?spectrum antiviral acyclic nucleoside phosphonates: (S )?HPMPC and (S )?HPMPA. Two alternate strategies allowing synthesizing selected amino acid, dipeptide, or ethylene glycol?linked amino acid prodrugs of (S )?HPMPC and (S )?HPMPA in solution and using

Guanidine Hydrochloride Purification of Proteins From SDS

Pepstatin A (2.0 mg/ml)10 ml Leupeptin (1.0 mg/ml)10 ml Aprotinin (5.0 mg/ml)(Protease Inhibitor Cocktail Sigma #P8340)Denaturation Buffer6 M guanidine hydrochloride 57.3 g G-HCl20 mM Hepes 7.9 2 ml 1M Hepes pH 7.910 mM KCl 0.074 g KCl0.5 mM DTT 100 ml 0.5 M

Guanidine Hydrochloride Purification of Proteins From SDS PAGE

(Sigma #P7626) 10 ml Pepstatin A (2.0 mg/ml) 10 ml Leupeptin (1.0 mg/ml) 10 ml Aprotinin (5.0 mg/ml) (Protease Inhibitor Cocktail Sigma #P8340) Denaturation Buffer 6 M guanidine hydrochloride 57.3 g G-HCl 20 mM Hepes 7.9 2 ml 1M Hepes pH 7.9 10 mM