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PRT PHOSPHATASE DILUTION BUFFER 1
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文献和实验. or more. Block in 30 ml of 1X Western buffer (containing 0.1% Tween-20 and 0.2% I-Block), gently rocking, 1 hr, room temperature. Add appropriate dilution of primary antibody (typically 1:5000 or 1:10,000) prepared in 1X Western buffer (containing
for 6 months without reducing binding activity. 2) PBS can not be used to dilute Streptavidin-AP since phosphate inhibit alkaline phosphatase activity. Fluorescent Dye (Avidin-FITC, Avidin-Texas Red, Avidin-AMCA) Dilution Buffer:
ELISA Procedure for Measuring Serum Antibody Titer
whole molecule/alkaline phosphatase conjugate p-Nitrophenyl phosphate Sodium carbonate buffer, 0.05 M pH 9.8, containing 1 mM magnesium chloride (MgCl2) Sodium hydroxide (NaOH), 2 M Procedure for Alkaline Phosphatase Detection1. Prepare a dilution of the goat
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