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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
Thermo Scientific™ Oxoid™
- 规格:
500g
Urea Agar Base (Dehydrated)
相关应用:
Industrial Microbiology
Detect rapid urease activity of the Proteae and non-rapid urease activity of some Enterobacteriaceae with Thermo Scientific™ Oxoid™ Urea Agar Base (Dehydrated).
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描述
Urea Agar Base is recommended for the preparation of Christensen medium1 for the detection of rapid urease activity of the urease-positive Proteae. The urea medium may be used for the detection of urea hydrolysis by some other Enterobacteriaceae but the incubation period is much longer 24-48 hours.
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For Laboratory Use Only
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文献和实验prior to the staining step. Failure to do so may cause a large amount of streaking and smearing at the base of SDS-PAGE gel. We usually fix our gels initially in methanol/acetic acid/water for at least 45 mins to remove them. Interfering Substances. a Lipids b
RNase and DEPC Treatment: Fact or Laboratory Myth
alone does indeed inactivate a substantial amount of RNase A (Figure 1). Various concentrations of RNase A were added to PBS and autoclaved. Aliquots of each solution were mixed with a 304 base 32 P-labeled RNA probe and incubated at 37°C for one hour, followed
for SD buffer to work, as like many chemical reactions time is required for completion, especially reduction of proteins with DTT. This is best carried out on the bench at room temperature, don't put it on ice, it'll take longer and the urea in the buffer
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