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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
二年
- 英文名:
α-Glucosidase
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
9001-42-7
- 规格:
100U/250U/1ku
| 规格: | 100U | 产品价格: | ¥388.0 |
|---|---|---|---|
| 规格: | 250U | 产品价格: | ¥780.0 |
| 规格: | 1ku | 产品价格: | ¥2280.0 |
α-葡萄糖苷酶,50units/mg protein 酵母
产品介绍
提取来源:酵母。
活力:≥50units/mg protein。
活力定义:One unit will liberate 1.0 μmole of D-glucose from p-nitrophenyl α-D-glucoside per min at pH 6.8 at 37℃。
性状:粉末。
使用注意事项:溶液保存,酶活是损失比较快的。
1、母液浓度尽量大于100u/ml,分装保存;
2、不要用纯水溶解,用PH7.0 200mM PBS缓冲盐溶液;
3、溶液不要在室外放置太久,最好一直置于2-8度环境下;
4、溶液不要冷冻,解冻过程对酶活损失影响很大。PNPG有很多种,所对应的酶是不一样的。 底物错误会导致不显色
| 储存条件: | -20℃ |
|---|---|
| 注意: | 部分产品我司仅能提供部分信息,我司不保证所提供信息的权威性,仅供客户参考交流研究之用。 |


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- 作者
- 内容
- 询问日期
文献和实验1. [IF=9.8] HaiNa Yuan et al."Structural and surface hydrophobic remodeling of whey protein-linoleic acid-EGCG complex suppresses co-oxidation, attenuates allergenicity, and enhances bioactive functionalities under oxidative stress."FOOD CHEMISTRY.2025 Nov;493:145950
2. [IF=9.8] Mengjia Zhu et al."Identification and characterization of novel α-glucosidase inhibitory peptides from sweet potato protein through heating combined with high hydrostatic pressure-assisted enzymatic hydrolysis."FOOD CHEMISTRY.2025 Aug;:145877
3. [IF=8.2] Qianwei Qu et al."Phyllanthus emblica L. fruit as potential functional ingredient: From optimized extraction to biological activities evaluation."Food Chemistry-X.2025 Jul;29:102840
4. [IF=8.2] Guohuo Wu et al."Comparative analysis of metabolite signatures and hypoglycemic effects in Toona sinensis leaves processed by two distinct methods."Food Chemistry-X.2025 Jul;29:102821
5. [IF=8.2] Jiaying Zhu et al."Metabolomics-based analysis of changes in functional constituents and activities of Tartary buckwheat by solid-state fermentation with three kinds of edible-medicinal fungi."Food Chemistry-X.2025 Jul;:102737
6. [IF=8] Peng Zhou et al."Evaluation of α-glucosidase inhibitory ability: Insights from free and bound polyphenols in five grain brans."FOOD RESEARCH INTERNATIONAL.2025 Jun;:116837
7. [IF=8.2] Qiong Wu et al."Fermentation-synergized physical modification: Enhancing physicochemical properties and bioactivities of soluble dietary fiber from peanut shells."Food Chemistry-X.2025 Jun;29:102667
8. [IF=8.5] Heping Hui et al."Optimization of microwave-assisted extraction for Herba Patriniae polysaccharide and its impact on physicochemical properties, antioxidant and hypoglycemic capacities: Primary structure-activity relationship."INTERNATIONAL JOURNAL OF BIO
9. [IF=8.5] Jun Qin et al."Degradation polysaccharide of Euryale ferox Salisb. Seeds significantly enhanced the hypoglycemic efficiency of type 2 diabetes mellitus."INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES.2025 Jul;318:145238
10. [IF=9.8] Di Zhao et al."Potential of different fractions of polyphenols in persimmon peels: Phenolic profiles, bio-activities, and mechanism of inhibition against α-glucosidase."FOOD CHEMISTRY.2025 Nov;492:145373
11. [IF=9.8] Chen Chen et al."Molecular networking-based discovery of components with antioxidant and α-glucosidase inhibitory activities in burdock root."FOOD CHEMISTRY.2025 Jun;:145278
12. [IF=10.2] Huihui Sun et al."Red-emissive carbon dot-cobalt oxyhydroxide nanosystem: A turn-on sensor for α-Glucosidase activity and inhibitor identification."Materials Today Bio.2025 Aug;33:102018
正常参考值:8--53U/L; 任意尿液:0--370U/L!答:两者方法学上实际上都属于麦芽七糖法。区别主要是所采用的底物有所不同,ESP-G7的优点在于其底物是采用亚乙基将麦芽糖苷的非还原端封闭,这可以阻止α-葡萄糖苷酶对底物的水解作用,提高底物的稳定性。EPS-G7:4,6-亚乙基-a,D-麦芽七糖苷-对硝基苯酚PNP-G7:对硝基苯麦芽糖庚酰。
-CUP比G3-pNP的催化速度快10倍,从而使G3-CNP可用为α-淀粉酶的底物。根据IFCC所提出的理想的测定方法条件:要求不使用辅助酶,反应产物具有确定的结构,产物组成恒定等,G3-CNP或Gal-G2-CNP更符合这一要求。Gal-G2-α-CNP的结构式如下:↑所示为淀粉酶作用部位,而α葡萄糖苷酶在此部位无水解作用。 α-葡萄糖苷酶能水解G≤3的麦芽寡精,但非还原性末端用半乳糖代替后则不能水解,即内源性葡萄苷酶对Gal-G2-α-CNP无水解作用。本文实验在试剂中
药物可刺激内源性insulin分泌,增强insulin的作用。用tolbutamide无效病人,改用chlorpropamide、glibenclamide等仍有效。应注意,起初治疗有效的病例,经6~12个月后约有10%~15%病人突然丧失疗效,原因未明。对继发性失效者可加用biguanides或α-葡萄糖苷酶抑制剂等作联合治疗,大多数病人最终需用insulin治疗。 应根据病人及药物特点选择sulfonylureas药物。一般中年的轻、中度糖尿病宜选用tolbutamide
技术资料





