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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8℃
- 英文名:
Atractylenolide III
- 库存:
88
- 供应商:
上海源叶生物科技有限公司
- CAS号:
73030-71-4
- 规格:
20mg
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文献和实验Polymerase III in vitro Transcription
Polymerase III in vitro Transcription Steve Hahn For the following reactions, use appropriate shielding and dispose of radioactive waste properly! A 20 microliter transcription reaction contains: 4.0 μl 5X Pol III transcription buffer 0.2 μl
Nested Deletions Using Exonuclease-III and Mung Bean Nuclease
- 0.1% triton X-100 - 50% glycerol Comments : The DNA must be >85% supercoiled. ExoIII will digest from nicks. Use CsCl
Pol III whole cell yeast extract (small scale)
Pol III whole cell yeast extract (small scale) Mike Schultz, Pavel Aprikian, Steve Hahn last modified6/28/99 Grow 2 liters yeast in YPD (with 3% glucose) to an A600 of 1.5 to 2.0 (inoculate ~1.5 ml saturated overnight








