重组人UBE2L3(带His标签)优惠促销

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产品名称：重组人UBE2L3(带His标签)
英文名称：Recombinant Human Ubiquitin Conjugating Enzyme E2 L3, His
产品货号：GS4893
产品规格：10μg

分子量：Approximately 18.9kDa,a single non-glycosylated polypeptide chain containing 154 amino acids(a.a.)of human UBE2L3/UBCH7 and 8 a.a.vector sequence including 6×His tag at N-terminus.
储存条件：冷冻（-20℃）

概述

Ubiquitin-conjugating enzyme E2 L3 belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2L3 gene in humans.The ubiquitin-conjugating enzymes,also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes,take part in the second step in the ubiquitination reaction.In this reaction,E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue.The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region.The UBE2L3 specifically acts with HECT-type and RBR family E3 ubiquitin-proteinshown to interact with UBOX5,ARIH1,Cbl gene,UBE3A and NEDD4.Down-regulated during the S-phase it is involved in progression through the cell cycle.Additionally,UBE2L3 regulates nuclear hormone receptors transcriptional activity and plays a role in myelopoiesis.

使用说明

This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.

技术规格
 

外观	Sterile Filtered White lyophilized(freeze-dried)powder.
纯度	＞95%by SDS-PAGE and HPLC analyses.
来源	Escherichia coli.
生物活性	Data is not available.
成分	Lyophilized from a 0.2μmfiltered concentrated solution in PBS,1mMDTT,pH 7.5.
内毒素	Less than 1 EU/μg of rHuUBE2L3,His as determined by LAL method.
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/ml.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions.
氨基酸序列	MHHHHHHAMA ASRRLMKELE EIRKCGMKNF RNIQVDEANL LTWQGLIVPD NPPYDKGAFR IEINFPAEYP FKPPKITFKT KIYHPNIDEK GQVCLPVISA ENWKPATKTD QVIQSLIALV NDPQPEHPLR ADLAEEYSKD RKKFCKNAEE FTKKYGEKRP VD

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