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- 详细信息
- 文献和实验
- 技术资料
- 库存:
116
- 英文名:
Recombinant Human Keratinocyte Growth Factor-2/FGF-10
- 保质期:
长期
- 供应商:
北京百奥莱博科技有限公司
- 保存条件:
冷冻(-20℃)
- 规格:
5μg
特别提示:包括重组人FGF-10/KGF-2在内,本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!
产品名称:重组人FGF-10/KGF-2
英文名称:Recombinant Human Keratinocyte Growth Factor-2/FGF-10
产品货号:GS4837
产品规格:5μg
分子量:Approximately 19.1kDa,a single,non-glycosylated polypeptide chain containing 169 amino acids.
储存条件:冷冻(-20℃)
概述
Fibroblast growth factor 10 belongs to the fibroblast growth factor(FGF)family which is involved in a variety of biological processes such as embryonic development,cell growth,morphogenesis,tissue repair,tumor growth and invasion.Like most other FGF family members,FGF-10 also has a heparin-binding domain and it plays an important role in the regulation of embryonic development,cell proliferation and cell differentiation.In addition,FGF-10 may play a role in wound healing and is required for normal branching morphogenesis.Recombinant human FGF-10 contains a 208 amino acids and it shares 92%and 95%amino acid sequence identity with murine and rat FGF-10.Defects in FGF-10 are the cause of autosomal dominant aplasia of lacrimal and salivary glands and lacrimo-auriculo-dento-digital syndrome.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
| 外观 | Sterile Filtered White lyophilized(freeze-dried)powder. |
| 纯度 | >97%by SDS-PAGE and HPLC analyses. |
| 来源 | Escherichia coli. |
| 生物活性 | Fully biologically active when compared to standard.The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5ng/ml,corresponding to a specific activity of>2.0×106 IU/mg. |
| 成分 | Lyophilized from a 0.2μmfiltered concentrated solution in 2×PBS,pH 7.4. |
| 内毒素 | Less than 1 EU/μg of rHuKGF-2/FGF-10 as determined by LAL method. |
| 复溶 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions. |
| 氨基酸序列 | LGQDMVSPEA TNSSSSSFSS PSSAGRHVRS YNHLQGDVRW RKLFSFTKYF LKIEKNGKVS GTKKENCPYS ILEITSVEIG VVAVKAINSN YYLAMNKKGK LYGSKEFNND CKLKERIEEN GYNTYASFNW QHNGRQMYVA LNGKGAPRRG QKTRRKNTSA HFLPMVVHS |
我公司销售的重组人FGF-10/KGF-2北京现货,Recombinant Human Keratinocyte Growth Factor-2/FGF-10,FGF-10/KGF-2, Human,重组Keratinocyte Growth Factor-2/FGF-10,重组人Keratinocyte Growth Factor-2/FGF-10质量上佳,价格普惠,欢迎广大新老客户踊跃订购。
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文献和实验/ml,不可振荡。 这个步骤即为溶解步骤,非常重要。 1) 一定要用推荐的溶液重悬(或溶解)冻干粉 用于溶解细胞因子或蛋白的溶液千差万别。此例中的重组人IL-4需用水溶解,而重组人IL-2 (产品编号:200-02)则需用100mM Acetic Acid (醋酸)溶解,重组人TGF-beta1 (产品编号:100-21)需用10mMCitric Acid (柠檬酸),pH3.0溶解,重组人FGF-basic(产品编号:100-18B)需用5mMTris,pH7.6溶解
-2 (产品编号:200-02)则需用100mM Acetic Acid (醋酸)溶解,重组人TGF-beta1 (产品编号:100-21)需用10mMCitric Acid (柠檬酸),pH3.0溶解,重组人FGF-basic(产品编号:100-18B)需用5mMTris,pH7.6溶解,重组人FGF-10(产品编号:100-26)需用5mMSodium Phosphate(磷酸钠),pH7.4溶解,重组IL-13(产品编号:200-13)需用20mMMHCl溶解。(注:即使同一重组细胞因子或蛋白,不同
要用推荐的溶液重悬(或溶解)冻干粉 用于溶解细胞因子或蛋白的溶液千差万别。此例中的重组人IL-4需用水溶解,而重组人IL-2 (产品编号:200-02)则需用100mM Acetic Acid (醋酸)溶解,重组人TGF-beta1 (产品编号:100-21)需用10mMCitric Acid (柠檬酸),pH3.0溶解,重组人FGF-basic(产品编号:100-18B)需用5mMTris,pH7.6溶解,重组人FGF-10(产品编号:100-26)需用5mMSodium
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