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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Blasticidin S (hydrochloride)
- 库存:
1
- 供应商:
北京博蕾德生物科技有限公司
- 规格:
10 mg
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文献和实验Guanidine Hydrochloride Purification of Proteins From SDS
Pepstatin A (2.0 mg/ml)10 ml Leupeptin (1.0 mg/ml)10 ml Aprotinin (5.0 mg/ml)(Protease Inhibitor Cocktail Sigma #P8340)Denaturation Buffer6 M guanidine hydrochloride 57.3 g G-HCl20 mM Hepes 7.9 2 ml 1M Hepes pH 7.910 mM KCl 0.074 g KCl0.5 mM DTT 100 ml 0.5 M
Guanidine Hydrochloride Purification of Proteins From SDS PAGE
(Sigma #P7626) 10 ml Pepstatin A (2.0 mg/ml) 10 ml Leupeptin (1.0 mg/ml) 10 ml Aprotinin (5.0 mg/ml) (Protease Inhibitor Cocktail Sigma #P8340) Denaturation Buffer 6 M guanidine hydrochloride 57.3 g G-HCl 20 mM Hepes 7.9 2 ml 1M Hepes pH 7.9 10 mM
Inducible shRNA Lentiviral Vectors
of One Shot® cells and mix gently. 3) Incubate the vial(s) on ice for 30 minutes. 4) Heat-shock the cells for 45 seconds at 42 °C without shaking. 5) Remove the vial(s) from the 42 °C water bath and place them on ice for 2 minutes. 6)
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