DMPO

EPR Spin-Trapping and Nano LC MS/MS Techniques for DEPMPO/OOH and Immunospin-Trapping with Anti-DMPO Antibody in Mitochondrial Electron Transfer Syste

in mitochondria. O2 •– generation by ETC also induces oxidative damage with protein radical formation. Immunospin-trapping with anti-DMPO antibody and subsequent mass spectrometry are used to define the specific site of oxidative damage, indicating cysteine-206

超氧化物歧化酶(SOD)的检测方法

自旋共振（ESR）光谱法 在室温下，溶液中O2-的ESR信号不能直接被检测出，但能够通过自旋捕捉法间接获得。最常见的诱捕剂是5,5-dimethyl-1-pyrroline N-oxide(DMPO)。由于O2- 诱捕DMPO会产生一个特殊的光谱图，因此ESR法是O2-检测的方法中特异性最好的一种。然而，DMPO与O2-间的二阶速度常数比O2-自发反应常数相对要低，因此需要向溶液中加入大量的DMPO（如：终浓度为0.45M）。这样，每次试验所要用的DMPO将花去大笔的经费。 这种方法的另一

Hydroxyl and 1-Hydroxyethyl Radical Detection by Spin Trapping and GC-MS

by spin trapping and ESR procedures often has been done using 5,5-dimethylpyrroline N-oxide (DMPO) as the spin trap (5 –9 ). This spin trap very rapidly reacts with OH to produce a stable free radical adduct having a characteristic ESR spectrum (5 –9 ).