Thrombin is a "trypsin-like" serine protease protein.Prothrombin(coagulation factor II)is proteolytically cleaved to form thrombin in the coagulation cascade,which ultimately results in the stemming of blood loss.As a result of cleaving,thrombin has two chains:a light chain(A chain,MW~6kDa)and a heavy chain(B chain,MW~31 kDa).These two chains are joined by one disulfide bond.Functions of thrombin are that it converts soluble fibrinogen into insoluble strands of fibrin,as well as catalyzes many other coagulation-related reactions.Thrombin also contains g-carboxyglutamyl residues.These modified glutamyl residues are the result of carboxylation by a microsomal enzyme,vitamin Kdependent carboxylase.g-Carboxyglutamyl residues are necessary for the Ca2+-dependent interaction with a negatively charged phospholipid surface,which is essential for the conversion of prothrombin to thrombin.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
外观
Sterile Filtered White lyophilized(freeze-dried)powder.
来源
Procine Blood
成分
Lyophilized Powder from 20mMPBS,pH 7.4 containing 0.9%NaCl.
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile water to a concentration of 10-100 u/ml.Stock solutions should be apportioned into working aliquots and stored at -20 ℃.Fur
Notice
1.Stable at neutral pH;2.Avoid keeping with heavy metal;3.Recommended to aliquot the solutions in plastic tubes.
Optimal Cleavage Sites
1.A-B-Pro-Arg-||-X-Y where A and B are hydrophobic amino acids and×and Y are nonacidic amino acids;2.Gly-Arg-||-Gly
Unit Definition
One unit is defined as the amount of enzyme needed to cleave 1mgof fusion protein in 16hoursto 95%completion at 20℃ in a buffer containing 25 mM Tris-HCl,pH 8.4,150 mM NaCl,and 2.5 mM CaCl2.