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试剂盒组分：
15 ml ImmPRESS (Peroxidase) Polymer Anti-Rabbit IgG Reagent (made in horse, ready-to-use)
15 ml 2.5% Normal Horse Serum for blocking (ready-to-use)
15 ml BLOXALL Endogenous Enzyme Blocking Solution
ImmPACT DAB EqV Substrate (7.5 ml Reagent 1 + 7.5 ml Reagent 2)
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文献和实验All-in-One Kit for Immunohistochemical Staining for Tissues with Antibodies
for more details. The HRP Polymer Conjugate will react with mouse, rabbit, guinea pig, and rat primary antibodies. 1) Dilute primary antibody in Antibody Diluent as recommended by the antibody manufacturer or as determined by a titration experiment
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
4 Antibody 22. Rabbit Anti-SeV Antibody 23. Alexa Fluor® 488 Goat Anti-Mouse IgG (H L) Antibody 24. Alexa Fluor® 594 Goat Anti-Mouse IgG (H L) Antibody 25. Alexa Fluor® 488 Goat Anti-Rabbit IgG (H L) Antibody 26. Alexa Fluor® 594
150µl of working dilution. Incubate sections in a humid chamber for 1 hour, RT. Blot off antibody, wash 2 times 5 min. each in 1x PBS. Prepare the working dilution of the secondary antibody (Horse anti-mouse IgG-Vector Catalog No. BA2001) in 1.0%
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