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- 11-223-1N
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- 2026年01月11日
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11-223-1N
货号：11-223-1N
规格：50L
产品名称：DPBS, powder, no calcium, no magnesium 干粉
Product Overview
Dulbecco's Phosphate Buffered Saline (DPBS) is intended to provide a buffer system for maintaining cell culture media in the physiological range of 7.2– 7.6. It generally serves as an isotonic saline solution or buffer for washing cells and tissues. It's utility in maintaining mammalian cells when a chemically-defined, balanced salt solution provides the appropriate milieu to sustain the physiological and structural integrity (i.e. tonicity/viability) of cells in vitro for a limited period of time. Biological Industries offers a range of DPBS formulations.
DPBS without calcium (Ca2+) and magnesium (Mg2+) is often used in trypsin solutions. DPBS without calcium and magnesium is used in the dissociation process to wash and resuspend cells when the presence of calcium and magnesium may inhibit trypsin activity.
This DPBS is modified as follows:
Without:
Calcium
Magnesium
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文献和实验The Cell Environment (including types of culture medium)
PBS, Hanks BSS, Earles saltsBalanced salt solutions DPBS (Prod. No. D8537 / D8662) Form the basis of many complex media HBSS (Prod. No. H9269 / H9394) EBSS (Prod. No. E2888)Basal media
generated JFH‐1 HCVcc ( protocol 3 ) Dulbecco's phosphate buffered saline (DPBS) without calcium and magnesium (see recipe in appendix 2A ; prepare with tissue‐culture‐grade H 2 O
The Cell Environment (including types of culture medium)
Examples Uses Balanced salt solutions PBS, Hanks BSS, Earles salts DPBS (Prod. No. D8537 / D8662 ) HBSS (Prod. No. H9269 / H9394 ) EBSS (Prod. No. E2888 ) Form the basis
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